Ocean Optics Curie UV-VIS Emission - Manual (Page 25)

Chapter 3
Using the Curie System
Introduction
This chapter provides you with instructions for performing experiments using the Curie system with the
OOIBase32 application.
Performing Experiments with the Curie System
The Curie system allows you to detect picomolar-range concentrations of fluorophores in solution with
emission from 200–850 nm.
Preparing for Experiments
The following procedures walk you through the steps necessary to carry out a fluorescence measurement
with your Curie system and OOIBase32 software.
Before performing an experiment, ensure the following:
• Both the Curie system and the OOIBase32 application with Curie custom software have been
correctly installed.
• The Wavelength Calibration Curie system configurations in OOIBase32 are correct. The
calibration coefficients (read from a memory chip in the USB2000 spectrometer) must match
the coefficients listed on the Wavelength Calibration data sheet that came with your
spectrometer (see
Spectrometer Configuration Dialog Box , Step 2 ).
• Both the Master and Channel Enabled options are enabled (see Spectrometer Configuration
Dialog Box , Step 3 ). The First Coefficient, Second Coefficient, Third Coefficient, and
Intercept should correspond to those of your Curie system.
• The minimum integration time is set to 20 milliseconds or greater. If you set the integration
time below 20 milliseconds, the lamp is driven too fast and the spectrum is more unstable.
• The A/D Interface settings are correct (see Spectrometer Configuration Dialog Box , Steps 4
and 5 ).
• Your Curie is plugged in and turned on (the switch on the end of the Curie is in the up or on
position).
Once you have installed and configured your hardware and software, and have set up your system, you
are ready to take a fluorescence measurement.
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