Ocean Optics Curie UV-VIS Emission - Manual (Page 34)

3: Using the Curie System
Curie VIS LVF 500 nm
Once you have chosen your excitation wavelength range, make a note of the location on the LVF scale
and lock the filter in place with the thumbscrew. Before making your fluorescence measurement, switch
back to Relative Irradiance mode by clicking on the on the OOIBASE toolbar.
Measuring Fluorescence with your Curie System
After you have chosen your excitation wavelength range, you are ready to make your measurements in
Relative Irradiance mode. Remove the CVD-DIFFUSE from the cuvette holder, place the software in
Relative Irradiance mode by clicking on the on the OOIBASE toolbar and place your sample in the
cuvette holder. Depending on the spectrum that you see, the data acquisition parameters and filtering can
be adjusted to provide optimal data.
Application Tips
If the signal you collect . . . You can . . . By . . .
Saturates the spectrometer
(peaks are off the scale) Decrease the light level
on scale in Scope mode • Decreasing the integration time, or
• Incorporating LVFs into your experiment
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