Ocean Optics Curie UV-VIS Emission - Manual (Page 47)

Appendix B
Calibrating the Wavelength of
the Curie Spectrometer
Your Curie system’s spectrometer is calibrated before it leaves Ocean Optics and the values are on the
CD that you received with your product. However, the wavelength for all spectrometers will drift slightly
as a function of time and environmental conditions, requiring you to recalibrate.
About Wavelength Calibration
You are going to be solving the following equation, which shows that the relationship between pixel
number and wavelength is a third-order polynomial:
λ
p
= I + C
1
p + C
2
p
2
+ C
3
p
3
Where: λ = Wavelength of pixel
p
I
= Wavelength of pixel 0
C
1
= First coefficient (nm/pixel)
C
2
= Second coefficient (nm/pixel 2 )
C
3
= Second coefficient (nm/pixel 3 )
You will be calculating the value for
I
and the three Cs.
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