Ocean Optics Curie UV-VIS Emission - Manual (Page 26)

3: Using the Curie System
Performing a Fluorescence Experiment
You can take a fluorescence reading in either Relative Irradiance mode (I) or Scope mode (S).
Relative Irradiance mode – Relative Irradiance mode is the preferred mode for fluorescence experiments.
Relative irradiance spectra are a measure of the intensity of a light source relative to a reference emission
source. There are two methods of performing a fluorescence experiment in Relative Irradiance mode: use
the custom Curie software provided with your Curie system to start OOIBASE32 in Relative Irradiance
mode using the calibration files generated during factory calibration, or perform your own radiometric
calibration using a blackbody of known color temperature. See Appendix C,
Relative Irradiance Mode ,
for more information.
• Scope mode – Scope mode is the preferred mode for choosing the excitation wavelengths with
the linear variable filters and configuring your acquisition parameters. The signal graphed in
Scope mode is the raw voltage coming out of the A/D converter. This spectral view mode
provides complete control of signal processing functions before taking absorbance, transmission,
reflection, and relative irradiance measurements. This mode reflects the intensity of the light
source, the reflectivity of the grating and mirrors in the spectrometer, the response of the detector,
and the spectral characteristics of the sample. Use Scope mode when configuring your setup,
adjusting the integration time, and choosing your excitation wavelengths with linear variable
filters.
An example of Curie spectra for quinine sulfate measured in Scope mode (S) and Relative Irradiance
mode (I) is shown below.
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