Olympus IX2-DSU BX-DSU User Manual
Page 45
VARIETIES OF OBSERVATION METHODS
/ Visual observation (IX)
II.
DSU
II .
4 - 5
Page
4-2-2 Simultaneous observation of reflected-light fluorescence and differential
interference (transmitting)
Differential interference observation requires the following units.
(1) One of condenser, IX2-LWUCDA, IX2-LWUCD or U-UCD8.
(2) One of differential interference slider, U-DICT, U-DICTS, U-DICTHC or U-DICTHR
(3) Analyzer, U-ANT or IX2-AN
The analyzer IX2-AN for simultaneous observation is put in lower
insertion port below the dichroic mirror in order to use reflected-light
fluorescene effectively.
If analyzer (U-ANT) is put in diffrential interference slider, the fluorescent
image may get dark and, also the analyzer may be burnt.
1. Put dummy mirror unit (the place where fluorescent mirror unit is not installed) into
light path.
2.
Adjust polarizer of universal condenser and make it in Cross-Nicol state.
3.
Insert revolving nosepiece into differential interference slider.
4. Turn turret of condenser and select turret of Nomarski prism that meets with
objective lens to be used.
5.
Put the objective lens to use into light path.
6.
Set specimen on stage and bring it in focus.
7.
Adjust field iris diaphragm and aperture of condenser.
8. Turn prism knob of differential interference slider and adjust contrast of differential
interference image.
9.
Put mirror unit to observe excitation desired into light path and open shutter.
10. Adjust transmitting light intensity so as to get optimum brightness for fluorescent
and differential interference image.
When change of reflected-light fluorescence and transmitting Nomarski
differential interference is frequently done but simultaneous observation
is not required, it would be convenient if mirror unit (IX2-MDICT) for
differential interference is attached at front side turret, instead of using
analyzer (IX2-AN). If the fluorescent mirror unit is changed to differential
interference mirror unit, the analyzer comes into light path together so that
the changeover is easy.
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