Olympus IX2-DSU BX-DSU - Manual (Page 42)

VARIETIES OF OBSERVATION METHODS
/ Visual observation (BX)
II. DSU
II . 4 - 2 Page
4-1-2 Simultaneous observation of reflected-light fluorescent and transmitting
Nomarski differential interference
Transmitting Nomarski differential interference observation requires the following units.
(1) Universal condenser (U-UCD8)
(2) Transmitting DIC slider (U-DICT, U-DICTS, U-DICTHR or U-DICTHC)
(3) Analyzer (U-AN or U-AN360-3)
(4) Revolving nosepiece or motorized revolving nosepiece for DIC.
The analyzer (U-AN or U-AN360-3) for simultaneous observation is put in
upper insertion port above the dichroic mirror in order to use reflected-
light fluorescene effectively.
If analyzer (U-ANT) is put in transmitting DIC slider, the fluorescent image
may get dark and, also the analyzer may be burnt.
1. Put dummy mirror unit (the place where fluorescent mirror unit is not installed) into
light path.
2. Adjust polarizer of universal condenser and make it in Cross-Nicol state.
3. Insert revolving nosepiece into transmitting DIC slider.
4. Turn turret of universal condenser and select turret of Nomarski prism that meets
with objective lens to be used.
5. Put the objective lens to use into light path.
6. Set specimen on stage and bring it in focus.
7. Adjust field iris diaphragm of transmitting illumination system (built-in the
microscope) and aperture of universal condenser.
8. Turn prism knob of transmitting DIC slider and adjust contrast of differential
interference image.
9. Put mirror unit to observe excitation desired into light path and open shutter.
10. Adjust transmitting light intensity so as to get optimum brightness for fluorescent
and differential interference image.
Regarding details of transmitting differential interference observation, see
manual of Universal condenser (U-UCD8).
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