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2 selection of fluorescent mirror unit – Olympus IX2-DSU BX-DSU User Manual

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DETAILS OF EACH OPERATION

/ Selection of fluorescent mirror unit

II.

DSU

II .

3 - 2

Page

Be careful that specimen of certain kind cannot use anti-color

deterioration agent.

3-2 Selection of fluorescent mirror unit

Select fluorescent mirror unit that has excitation to meet with coloring matter.

Do not use fluorescent mirror unit in combination with brightfield

of view mirror unit (U-MBF3). U-MBF3 is very bright and it may

hurt your eyes. If for any reason the mix use of mirror units is

required, use U-MBFL3 that ND filter is built in or insert ND filter of

3%.

Note to excitation bandwidth

A few sets of excitation filters combined for different bandwidth are

available. Typically, a set of wide bandwith (W) is used but, in case of

the followings, the use of a separate set may be better.

(1) In case that brightness of fluorescence is dark

(B,G excitation only)

Change to super wide bandwidth (SW)

(In case of SWB2, fluorescence that comes from
substance other than fluorescent coloring matter may
become stronger.)

(2) In case fluorescence that comes from substance other than

fluorescent coloring matter is strong

Change to narrow bandwidth (N)

(Brightness of fluorescence becomes slightly darker.)

Note

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Note