2 selection of fluorescent mirror unit – Olympus IX2-DSU BX-DSU User Manual
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DETAILS OF EACH OPERATION
/ Selection of fluorescent mirror unit
II.
DSU
II .
3 - 2
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Be careful that specimen of certain kind cannot use anti-color
deterioration agent.
3-2 Selection of fluorescent mirror unit
Select fluorescent mirror unit that has excitation to meet with coloring matter.
Do not use fluorescent mirror unit in combination with brightfield
of view mirror unit (U-MBF3). U-MBF3 is very bright and it may
hurt your eyes. If for any reason the mix use of mirror units is
required, use U-MBFL3 that ND filter is built in or insert ND filter of
3%.
Note to excitation bandwidth
A few sets of excitation filters combined for different bandwidth are
available. Typically, a set of wide bandwith (W) is used but, in case of
the followings, the use of a separate set may be better.
(1) In case that brightness of fluorescence is dark
(B,G excitation only)
Change to super wide bandwidth (SW)
(In case of SWB2, fluorescence that comes from
substance other than fluorescent coloring matter may
become stronger.)
(2) In case fluorescence that comes from substance other than
fluorescent coloring matter is strong
Change to narrow bandwidth (N)
(Brightness of fluorescence becomes slightly darker.)
Note
TIP
Note