Details of each operation, 3 - 3 page – Olympus IX2-DSU BX-DSU User Manual
Page 27
DETAILS OF EACH OPERATION
/ Selection of fluorescent mirror unit
II.
DSU
II .
3 - 3
Page
Table of combination for mirror unit and dichroic mirror filter
Excitation
Method
Mirror Unit
Dichroic Mirror
Excitation
Filter
Barrier Filter
Fluorochrome
U-MWU2
BP330-385
U
U-MNU2
DM400
BP360-370
BA420
• Auto fluorescence observation
• DAPI Dyeing: DNA
• Hoechest 33258, 33342 Dyeing:
Chromosome
V
U-MNV2
DM455
BP400-410
BA455
• Catecholamine observation
• serotonin observation
• tetracycline Dyeing: Bone, Teeth
U-MWBV2
BP400-440
U-MNBV2
DM455
BP420-440
BA475
BV
U-MCFPHQ
DM450
BP425-445
BA460-510
•
quinacrine, quinacrine mustard
Dyeing: Chromosome
• thioflavine S Dyeing: lymph cell
• acriflavine Dyeing: Nucleic acid
• CFP use
U-MGFPHQ
DM485
BP460-480
BA495-540
U-MWB2
BP460-490
U-MNB2
BP470-490
B
U-MSWB2
DM500
BP420-480
BA520IF
U-MWIB2
BP460-490
U-MNIB2
DM505
BP470-490
BA510IF
IB
U-MYFPHQ
DM505
BP490-500
BA515-560
• FITC Dyeing: Fluorescent
antibody test
• acridine orange Dyeing:DNA, RNA
• auramine Dyeing: TB bacteria
• EGFP, S65T, RSGFP use
• YFP use
U-MWG2
BP510-550
U-MNG2
BP530-550
G
U-MSWG2
DM570
BP480-550
BA590
DSU-
MRFPHQ
DM560
BP535-555
BA570-620
IG
U-MWIG2
DM565
BP520-550
BA580IF
• rhodamine, TRITC Dyeing:
Fluorescent antibody test
• propidium iodide Dyeing: DNA
• RFP use
IY
U-MWIY2
DM600
BP545-580
BA610IF
texas red Dyeing: Fluorescent
antibody test