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Details of each operation, 3 - 3 page – Olympus IX2-DSU BX-DSU User Manual

Page 27

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DETAILS OF EACH OPERATION

/ Selection of fluorescent mirror unit

II.

DSU

II .

3 - 3

Page

Table of combination for mirror unit and dichroic mirror filter

Excitation

Method

Mirror Unit

Dichroic Mirror

Excitation

Filter

Barrier Filter

Fluorochrome

U-MWU2

BP330-385

U

U-MNU2

DM400

BP360-370

BA420

• Auto fluorescence observation
• DAPI Dyeing: DNA
• Hoechest 33258, 33342 Dyeing:

Chromosome

V

U-MNV2

DM455

BP400-410

BA455

• Catecholamine observation
• serotonin observation
• tetracycline Dyeing: Bone, Teeth

U-MWBV2

BP400-440

U-MNBV2

DM455

BP420-440

BA475

BV

U-MCFPHQ

DM450

BP425-445

BA460-510

quinacrine, quinacrine mustard

Dyeing: Chromosome

• thioflavine S Dyeing: lymph cell
• acriflavine Dyeing: Nucleic acid
• CFP use

U-MGFPHQ

DM485

BP460-480

BA495-540

U-MWB2

BP460-490

U-MNB2

BP470-490

B

U-MSWB2

DM500

BP420-480

BA520IF

U-MWIB2

BP460-490

U-MNIB2

DM505

BP470-490

BA510IF

IB

U-MYFPHQ

DM505

BP490-500

BA515-560

• FITC Dyeing: Fluorescent

antibody test

• acridine orange Dyeing:DNA, RNA
• auramine Dyeing: TB bacteria

• EGFP, S65T, RSGFP use
• YFP use

U-MWG2

BP510-550

U-MNG2

BP530-550

G

U-MSWG2

DM570

BP480-550

BA590

DSU-

MRFPHQ

DM560

BP535-555

BA570-620

IG

U-MWIG2

DM565

BP520-550

BA580IF

• rhodamine, TRITC Dyeing:

Fluorescent antibody test

• propidium iodide Dyeing: DNA

• RFP use

IY

U-MWIY2

DM600

BP545-580

BA610IF

texas red Dyeing: Fluorescent
antibody test