Hoefer IEF100 - Manual (Page 7)

• p1
Function and Description
The Hoefer IEF100 Isoelectric Focusing instrument is designed to
perform isoelectric focusing of proteins in Immobilized pH Gradient
(IPG) strips. It is an integrated system with a built-in Peltier cooled
temperature platform, and a 12,000 V, 10 W power supply capable
of delivering up to 10 mA. There are 30 fully editable protocols, nine
of which are pre-programmed with recommended IEF protocols. One
running tray will hold all currently available IPG strips, 3.0 – 3.5 mm
wide. The IPG strips are run gel side up, using electrode wicks to
help remove impurities that collect at the end of the pH gradients.
Samples can be loaded into the IPG strips during rehydration, or
with sample cups.
The IEF100 is fully tested and certified to all applicable international
standards.
Features of the IEF100 are:
• One 6 channel tray that focuses:
Up to 6, 7 – 24 cm IPG strips using the single electrode set.
Up to 12, 7 cm IPG strips using the dual electrode accessory
(included).
• Sample loading during rehydration or with sample cup.
• Monitors current in each IPG strip.
• Integrated 12,000 volt DC power supply, with 10 mA capability,
the most voltage and current capability available.
• A large blue/white LCD display for easy viewing that supports a
graphical display of voltage and current.
• The ability to program, edit and store up to 30 protocols, each
with 9 steps.
• Constant power mode.
• A real time clock, settable to local time zones.
• Temperature controlled platform.
• Ethernet and RS232 ports to report data or load protocols.
Note: Ohm’s law states that the
10 mA of output is available at
1000 V and less.
Fig. 1. The Hoefer IEF100.