Hoefer IEF100 User Manual
Page 29
• p23
Isoelectric Focusing
(using the Single Electrode Set)
1
Open the safety lid by pressing down on the white push label in the middle
of the front of the lid (Fig. 17).
2
Insert the IEF105 focusing tray on the far right side of the cold plate. The
tray has only one orientation. There are (+) and (–) marks on the tray that
align with corresponding marks on the IEF100.
The tray will not fit properly in the IEF100 in any other orientation.
3
Slide the focusing tray to the left under the clamping tabs (Fig. 18). These
tabs improve the contact, and the heat transfer, between the tray and the
cold plate.
4
Use forceps to load the rehydrated IPG strips into the focusing tray gel side
up. The anodic (+) end of the strips should be on the left, matching the (+)
on the tray and the IEF100 (Fig. 19).
The strip channels are numbered so that channel 1 is closest to the front
and channel 6 is at the back of the IEF100.
Fig. 18. Slide the focusing tray.
Fig. 17. Open the safety lid.
Fig. 19. Load the IPG strips.
Note: In any one run, all the IPG strips
should be the same length for the
electrodes to make good contact.