Hoefer IEF100 User Manual
Page 19
• p13
The lower case “m”, “u”, and “n” can be used to designate milli,
micro, and nano, if desired.
When the name is correct, click the knob to enter the name, and
move to the next field.
Delay
The delay line is an optional step. Both a time and a temperature
can be entered. Focusing will start after the delay time has elapsed.
This can be helpful for overnight runs where the user may want to
have focusing end at some specific time the next morning. The Peltier
modules will maintain the cold plate at the set temperature.
Parameter
Units
Range
Increment
Time
(hour:min)
0:00 – 99:59
0:15
Temperature
(°C)
15 – 25
1
IEF Limits
Maximum volt, current per strip, and watt per strip are applied to
the protocol. These settings will override the values in the individual
steps. These overall limits can be used to avoid certain focusing
conditions that cause damage or problems with the IPG strips.
The temperature for the focusing is also set. Since the isoelectric
points of proteins are temperature dependent, IEF is typically at
a single temperature, usually 20 °C. It is not recommended to use
temperatures lower than 20 °C as it can cause problems with urea
crystallization in the IPG strip.
Knob
Computer
Settable
Settable
Parameter
Units
Range
Increments
Increments
Volt
(V)
0 – 12,000
250
1
Current/strip
(μA)
0 – 999
25
1
Watts/strip
(W)
0 – 2.0
0.1
0.1
Temperature
(°C)
15 – 25
1
1
Note: Times to the nearest minute
increment can be uploaded to protocols
from a computer.
Note: It is recommended not to
exceed 6000 V or more than 0.5 W/
strip for a 7 cm IPG. Please follow the
IPG strip manufacturer’s instructions
for recommended limits.