Hoefer IEF100 - Manual (Page 62)

• p56
Agarose Overlay
1% Agarose in 1X Electrophoresis Buffer
Prepares 100 ml
1% agarose, 25 mM Tris, 192 mM glycine, 0.1% SDS
Prepare in a 500 ml flask to allow room for foaming.
Final Concentration Amount
Agarose 1% 1 g
10X Electrophoresis Buffer 1X 10 ml
(250 mM Tris, 1.92M Glycine, 1% SDS)
Bromophenol blue 3 mg
Deionized water to 100 ml
Gently swirl to suspend agarose.
Heat at low power in a microwave oven until agarose is fully dissolved.
Store in 1.5 ml aliquots at 4 °C in plastic screw top tubes.
Reheat aliquots in heating block.
Caution! SDS may cause the solution
to boil over so exercise caution when
heating and prevent boiling over.