Hoefer IEF100 User Manual
Page 21

• p15
Step Time
Steps can be programmed to run for a set amount of time, Hrs (00:00
in hours:minutes), or for a set amount of volt-hours, Vhrs (volts
multiplied by the number of hours). Most IEF strip manufacturers
recommend to run the IPG strips to a specific volt-hours endpoint.
The researcher should determine the endpoint for their specific sample.
The first step with a 0 value entered in the step time is treated as the
end of the protocol.
Step Value
Knob
Computer
Settable
Settable
Step Type
Range
Increments Increments
Constant Volt
0 – 12,000
250
1
Gradient Volt
0 – 12,000
250
1
Constant Watt
0.1 – 2.0
0.1
0.1
Step Time
Hrs (hours:minutes)
00:00 – 99:59
0:15
0:01
Vhrs (volt-hours)
0 – 300,000
500
1
Note: When switching between time
units, the IEF100 will convert between
hours and Vhrs automatically. It may
help to set the step time units (Hrs or
Vhrs) before the numerical endpoint
is entered.
- IEF100 (65 pages)
- SE900 (37 pages)
- SE260 (31 pages)
- SE260 (31 pages)
- SE300 miniVE (37 pages)
- SE300 miniVE (37 pages)
- SE300 miniVE (37 pages)
- SE410 (51 pages)
- SE410 (50 pages)
- SE640 (51 pages)
- SE640 (50 pages)
- SE275 (21 pages)
- SE245 (13 pages)
- SE675 (27 pages)
- SG100 (9 pages)
- SG500 (12 pages)
- SQ33 Sequencer (30 pages)
- SQ33 Sequencer (30 pages)
- SQ33 Sequencer (30 pages)
- HE33 (26 pages)
- HE33 (26 pages)
- HE99X (26 pages)
- HE99X (26 pages)
- HE-PLUS System (26 pages)
- HE-PLUS System (26 pages)
- SUB Series (31 pages)
- SUB Series (31 pages)
- TE70X (31 pages)
- TE70X (31 pages)
- PR648 (11 pages)
- PR150 (9 pages)
- PR645 Immunoblot XL (21 pages)
- TE22 (35 pages)
- TE22 (35 pages)
- TE62 (35 pages)
- TE22 (31 pages)
- DQ300 (30 pages)
- DQ300 (30 pages)
- UVIS20 (21 pages)
- UVIS20 (21 pages)
- SP-2001 Vision Life Spectrophotometer (66 pages)
- Single Channel Variable Pipette (8 pages)
- PR250 (18 pages)
- PP24 (10 pages)