Hoefer IEF100 - Manual (Page 35)

• p29
Sample Cups
Sample cups can be used to apply protein sample to IPG strips
if it was not included in the rehydration buffer. The sample cups
are supplied in strips of six cups. The sample cups can be used all
together, or cut apart and used separately.
1
Press the sample cups down on top of the IPG strips. The sample cups
should stop on the bottom of the channel and seal on the IPG strips
(Fig. 30).
In general, most cup loading is done on the anodic end of the IPG strip.
Each sample is different. In some situations, samples may focus better
loaded elsewhere on a strip. This can only be determined experimentally for
different sample types.
2
Up to 240 μl sample can be applied to the sample cups (Fig. 31).
3
Once loaded, confirm the protocol settings.
4
Close the lid (Fig. 32).
Note: There are small semicircular
features in the bottom of the tray
channels that help align the IPG strip
in the middle of the lane. The legs
of the sample cup must
not hit these
features or else the sample cup will
leak. See Fig. 21 on page 24.
Fig. 30. Place sample cups.
Fig. 31. Apply sample.
Fig. 32. Close safety lid.