Hoefer IEF100 User Manual
Page 30
• p24
5
Align the (+) end of the strips with the alignment mark in the tray (Fig. 20).
This will help in two ways. First, the clamping tabs will not interfere with the
positioning of the electrodes. Secondly, there are small grooves in the bottom
of the channels for forceps to remove IPG strips after IEF.
6
Apply electrode wicks on top of each end of the IPG strips, overlapping the
gel by 2– 3 mm, and extending off the end of the IPG strip.
• The electrode wicks are supplied in long perforated strips. Use scissors to
cut off the desired number of wicks.
• Moisten the IEF wicks with water and gently blot off any excess water.
Fig. 22. Wick placement.
Fig. 21. Focusing tray enlarged.
Grooves for Forceps
to Remove IPG Strips
IPG Strip
Alignment Line
IPG strip alignment features.
Do not use sample cup over
these features.
Clamping
Tabs
Fig. 20. Align strips.
Note: The electrode wicks absorb ions
collecting at the ends of the IPG strips,
and generally improve the IEF results.
Platinum Wire
Electrode Wick
IPG Strip