Hoefer IEF100 - Manual (Page 15)

• p9
Electrode Wicks
The electrode wicks are small rectangular filter papers that are placed
over the end of the IPG strips. The electrodes make contact with the
top of the wicks. The electrode wicks help remove impurities that
collect at the end of the IPG strips, and generally improve electrode
contact.
The electrode wicks come in long strips, and must be cut apart into
individual rectangles as they are used. The electrode wicks need to be
moistened by lightly blotting with water prior to use.
Sample Cups
The sample cups load protein into the IPG strip. Sample cups can
hold up to 240 μl of sample. The cups can be applied in most places
along the length of the IPG strip, and are normally applied at the
anode (+) end.
Rehydration/Equilibration Trays
The rehydration/equilibration trays can be used for both functions.
The lanes are narrow enough for proper rehydration, but deep
enough to hold the volume of reagent required for equilibration. A
small tray is included for use with 7 cm IPG strips. The large tray can
be used with IPG strips up to 24 cm in length.
Brush
The cleaning brush is used for cleaning the focusing tray.
Forceps
Forceps facilitate handling of IPG strips.
Note: The electrodes will not make
contact with the IPG strips without the
electrode wicks in place.
Note: The sample cups can be cut into
individual cups, if desired.
Fig. 8. Sample cups.
Fig. 9. Rehydration/equilibration trays.