ChemoMetec SP-100 - Manual (Page 50)

5 Operation of NucleoCounter SP-100
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5.18 Measuring (“Run” button)
When a cassette has been loaded and inserted into the instrument, the measurement is
activated, by pressing the “
Run” button. The display shows:
Display Text Type 30
Preparing Preparing Preparing Preparing
Figure 60. The instrument is in the “ preparing” mode
During ” preparing”, the actuator moves the piston rod down through the barrel of the
cassette. Hereby, the sample/dye mixture is transported to the cassette measurement
chamber. The form and dimensions of the flow channels facilitate effective mixing of
sample and stain. Sensors monitor sample flow. When the sample has been correctly
loaded into the measurement chamber, the piston is stopped.
Now the display shows:
Display Text Type 31
Analysing Analysing Analysing Analysing
Figure 61. The instrument is in the “analysis” mode
The analysis will occur after a few seconds and the fluorescent image is recorded. Once
recorded, the image is analysed by the instrument’s integral software (firmware). Upon
completion of the analysis, the result is shown on the display. The entire process takes
approximately 30 seconds. If an external computer is connected, the results and the
recorded fluorescent image can be viewed using SemenView software.
After each measurement the cassette can be safely removed (see Section 5.17 ).
Sometimes, however, the complete process described above may be interrupted. Please
familiarize yourself with
Section 8 Troubleshooting, Error messages .
Instruments with the Viability Option included
If the instrument is in the viability mode (F31 is On), then the following text is
displayed when the “
Run” bottum is activated: