ChemoMetec SP-100 - Manual (Page 38)

5 Operation of NucleoCounter SP-100
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5.5 Dilution Factor (F0)
The Instrument will automatically multiply the actual sperm count of a diluted semen
sample with the so-called Dilution Factor (DF). When testing a semen ejaculate from a
boar it is recommended to dilute the sample 201 times by adding 200 volumes of
Reagent S100 to 1 volume of sample. The instrument can compensate for this 201-fold
dilution using the DF feature and present the sperm count of the ejaculate.
EXAMPLE EXAMPLE EXAMPLE EXAMPLE - Changing the DF from 201 to 1000
To change the DF press “ F0 + ↵ ↵ ↵ ↵ ”. If the current DF is 201 the following will be
displayed:
Display Text Type 12
Dilution factor Dilution factor Dilution factor Dilution factor
DF=201 DF=201 DF=201 DF=201
Enter new DF + Enter new DF + Enter new DF + Enter new DF + ↵ ↵ ↵ ↵
New DF=_ New DF=_ New DF=_ New DF=_
Figure Figure Figure Figure 29 29 29 29. . . . The Dilution Factor can now be typed in
Then type “ 1000 + ↵ ↵ ↵ ↵ ” and the DF will be changed to 1000:
Display Text Type 2
SP SP SP SP- - - -100 100 100 100
Ready Ready Ready Ready
ID: ID: ID: ID:- - - - DF=1000 DF=1000 DF=1000 DF=1000
Figure Figure Figure Figure 30 30 30 30. . . . Dilution Factor is set to 1000
The DF must be a 1 to 4-digit whole number in the range from 1 to 9999. A DF value
below 11 is not recommended unless it is previously validated that the results are not
negatively affected. In particular it should be validated thoroughly if the buffer capacity
of the sample is so high that it may impact the overall pH of the lyzate mixture.
If the DF has been changed the new value will be stored until it is changed again. When
shutting down the SP-100 ask if the actual DF shall be saved as the new default value:
Display Text Type 8
Save parameters? Save parameters? Save parameters? Save parameters?
↵ ↵ ↵ ↵ : Save : Save : Save : Save
Esc: Cancel Esc: Cancel Esc: Cancel Esc: Cancel
Figur Figur Figur Figure e e e 31 31 31 31. . . . Shall the DF value be saved and used as the new default value?
Press “↵ ↵ ↵ ↵ ” to save or “ Esc” if the present default value should not be overwritten.
Please note, that the precision and accuracy is dependent on the total number of counts
in the cassette. Therefore, in order to obtain a high degree of precision and accuracy it