ChemoMetec SP-100 - Manual (Page 39)

5 Operation of NucleoCounter SP-100
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is important to adjust the Dilution Factor to the sperm cell density of the sample. See
Section 1.1.1 and Application Notes for more information on this subject.
Instruments with the Viability Option included
If the instrument is in the viability mode (F31 is On), then DF is replaced with DF1
(Dilution Factor for the Total Count) and DF2 (Dilution Factor for the Non-Viable Count).
With respect to performing a Total Count and a Non-Viable Count in the viability mode,
please refer to
“
Viability Testing with NucleoCounter SP-100, Addendum to User’ s Guide
(part no. 991-0104).
5.6 Correction Factor (F10)
The default value of the Correction Factor is 1000. This factor can be changed in order to
obtain a higher or lower cell count. The Correction Factor must be a number in the range
from 800 to 1200. A Correction Factor of 1050 means that all cell count results will be
multiplied with 1050/1000 = 1,050. This corresponds to a 5% increase in the cell count
levels.
The NucleoCounter SP-100 is calibrated from the factory but the “true” count of a
sample can always be questioned and likewise can it be discussed. Hence, the user may,
within certain limits, “correct” the NucleoCounter SP-100 result in order to match a
preferred reference method. However, it is recommended not to change the Correction
Factor unless there are particularly weighty grounds for doing this.
EXAMPLE EXAMPLE EXAMPLE EXAMPLE - Changing the Correction Factor from 1000 to 1050
To change the Correction Factor press “ F10 + ↵ ↵ ↵ ↵ ”. If the current Correction Factor is 1000
the following will be displayed:
Display Text Type 13
Corr. factor Corr. factor Corr. factor Corr. factor
CF=1000 CF=1000 CF=1000 CF=1000
Enter new CF + Enter new CF + Enter new CF + Enter new CF + ↵ ↵ ↵ ↵
New CF=_ New CF=_ New CF=_ New CF=_
Figure 32. The Correction Factor can now be typed in
Then type “ 1050 + ↵ ↵ ↵ ↵ ” and the Correction Factor will be changed to 1050. Or press “ Esc”
to jump back to the “ Ready” status without affecting the setting.
If the Correction Factor has been changed the new value will be stored until it is
changed again. When shutting down the SP-100 ask if the changes in the settings
including the actual Correction Factor shall be saved as the new default value: