BUCHI Encapsulator B-395 Pro - Manual (Page 65)

6 Operation
65 B-395 Pro Operation Manual, Version C
Special recommendations for cells
For dividing cells - dissolve the core alginate, then maintain Na/Ca-ratio >20:1 in the culture medium
so that the core will not re-solidify.
For resting cells – you can maintain the alginate core structure gelated and you can use even Ba 2+ , a
stronger gelating ion than Ca 2+ . Ba-alginate is extremely stable and withstands dissolution by 50 mM
citrate-solution for days.
Also see: Gröhn P. et al. 1994. Large-scale production of Ba 2+ alginate-coated islets of Langerhans for
immunoisolation. Exp. Clin. Endocrinol. 102: p.380-387.