BUCHI Encapsulator B-395 Pro - Manual (Page 60)

6 Operation
60 B-395 Pro Operation Manual, Version C
6 .11 Heat sterilization of the reaction vessel
1. Prepare the Reaction Vessel according to section 5.4.
2. Add 2 to 5 mL of water into the reaction vessel.
3. Verify that the following items are connected or prepared correctly:
• The proper sized nozzle
• The electrode centered below the nozzle
• Does the luer lock plug close the bead producing unit?
• Are the air and liquid filters attached?
• Is a magnetic stirrer bar in the reaction vessel?
• Is the bead collecting flask attached?
• Is the passage from the reaction vessel to the bead collecting flask open (open bead drain
valve)?
• Is the drain tubing closed with a clamp?
• Is the luer lock of the bead producing unit closed with a luer lock stopper?
4. Put the assembled reaction vessel in the autoclave and steam sterilize at 121°C for 20 minutes or
according to your protocol.
5. After autoclaving, remove the hot reaction vessel from the autoclave as soon as possible to avoid
water condensation in the air filter. Because a completely wetted air filter will no longer let air
pass, it will be difficult to drain the liquids from the reaction vessel, due to the negative pressure
created in the reaction vessel. To test the air filter’s condition, attach a 60 mL syringe to the filter.
When moving the syringe piston back and forth, only a slight resistance should be felt.
6. Close the bead collecting valve only after the reaction vessel has cooled down to room tempera-
ture.
6 .12 Sterilization of the pressure bottle
1. Assemble the pressure bottle and close the luer lock fitting with a luer lock stopper.
2. Add 1 to 2 mL of water into the pressure bottle. Place the assembled pressure bottle in the auto-
clave and steam sterilize at 121°C for 20 minutes or according to your protocol.