Dako Autostainer - Manual (Page 85)

Dako Autostainer | User Guide 85
Section 14 | Starting a Run
After the specimen-containing slides and reagent vials have been loaded, it is advisable to go through
the following checklist:
Slides
• should be flooded with buffer containing 0.05% Tween 20 using a squirt bottle to prevent
slides from drying while loading additional slides.
• should be seated securely in slide rack.
Racks
• should be properly seated in the Autostainer.
Reagents
• should be at room temperature.
• should be placed at proper locations in the reagent racks.
• should contain no air bubbles.
• should contain at least the reagent volume in each vial as specified in the
Reagent Layout Map.
Buffer
• reservoir should contain at least the volume of buffer indicated in the Reagent Layout Map
plus any additional volume listed for the completion of a delayed run.
Water
• reservoir should contain at least the volume of water indicated in the Reagent Layout Map.
Waste
• containers should have enough room to accommodate the volume of buffer
and water used during the staining run.
After the above checks have been made the Start Run sequence can be initiated.