Dako Autostainer - Manual (Page 83)

Dako Autostainer | User Guide 83
Section 13 | Loading Slides
After programming is completed and the reagents have been loaded, you are ready to load the slides
on the slide racks. Print the Slide Layout Map by selecting the PRINT button in the SLIDE LAYOUT
MAP:LOAD SLIDES screen. This map provides a guide for loading slides and can be saved as part
of your staining record.
Loading Microscope Slides
NOTE: The slides should soak in a buffer solution containing 0.05% Tween 20 for a minimum of 5
minutes prior to loading on the Autostainer.
1. Tilt the slide rack at a 30 ° angle away from you, allowing the back of the rack handles to
rest in the loading slots.
2. Hold the slide by placing your thumb and forefinger on each side of the slide’s unfrosted
end.
3. Load the slide into the slide rack by gently pushing the frosted end into position. The slide
is securely held to the rack. (see photo)
4. Secure the slide rack in the instrument by aligning the holes in the handles over the pins
in the sink frame. Gently flood the slides with buffer containing 0.05% Tween 20 using a
squirt bottle to prevent the slides from drying while loading additional slides.
Microscope slide
Slide Rack
Frosted end
of slide