Dako Autostainer - Manual (Page 104)

104 Dako Autostainer | User Guide
Reagent Preparation
One Liter of Tris-Buffered Saline Solution (S3001)
1. Add 1L (1000 mL) of deionized water to a 1L carboy, flask or beaker.
2. Empty entire contents of one packet. Use a magnetic stir plate to mix and dissolve the
TBS granules.
3. Add 0.5 mL of Dako Tween 20 (Code No. S1966) to the Tris-buffered saline solution and
mix using a magnetic stir plate.
4. The final solution is 50mM Tris-HCl, 150mM NaCl buffered saline, pH 7.6 with 0.05%
Tween 20.
Five Liters of Tris-Buffered Saline Solution (S1968)
1. Add 5 L (5000 mL) of deionized water to a 5 L carboy, flask or beaker.
2. Empty entire contents of one packet. Use a magnetic stir plate to mix and dissolve the
TBS granules.
3. Add 2.5 mL of Dako Tween 20 (Code No. S1966) to the Tris-buffered saline solution and
mix using a magnetic stir plate.
4. The final solution is 50mM Tris-HCl, 150mM NaCl buffered saline, pH 7.6 with 0.05%
Tween 20.
Ten Liters of Tris-Buffered Saline Solution (S3006)
1. Prior to dilution, allow the Wash Buffer 10X solution to reach room temperature (20-
25 °C).
2. Add 1L (1000mL) of concentrated Wash Buffer 10X solution to a 10L carboy, flask or
beaker.
3. Add 9L (9000mL) of deionized water to the same container. Use a magnetic stir plate to
stir gently for at least 15 minutes.
4. The final solution is 50mM Tris-HCl, 150mM NaCl buffered saline, pH 7.6 with 0.05%
Tween and a preservative.