Bio-Rad PROTEAN® i12™ IEF System - Manual (Page 9)

Chapter 1 PROTEAN IEF System
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1.2 Unpacking and Setup
1. Carefully inspect the shipping container for any damage that may have occurred during shipping. Severe
damage to a container may indicate damage to its contents. If you suspect damage to the contents,
immediately file a claim with the carrier in accordance with their instructions before contacting Bio-Rad
Laboratories.
2. Open the shipping carton and lift the content out of its packing. Inspect the instrument for external
damage. If any part is missing or damaged, contact Bio-Rad Laboratories immediately.
3. Place the PROTEAN i12 IEF cell on a firm, flat surface.
a. Position the cell so that there is access to the USB ports (back panel) and power switch (right panel).
b. To ensure adequate cooling, be sure that there is ≥6 cm clearance around the unit. DO NOT block the
fan vents.
c. Make sure the system is on a level surface. Place the leveling bubble in the center of the cooling
platform and adjust the instrument leveling feet as needed.
4. Connect the cell to a three-prong, grounded AC outlet using the power cord provided with the cell.
Do not place cloth or absorbent pads underneath the instrument. These or other loose
items may be pulled into the fan intake, causing damage to the unit due to overheating
and voiding the warranty.
1.3 Workflow
Figure 1.5 summarizes the workflow described in this manual. For best results, use the reagents and
protocols available in the ReadyPrep 2-D starter kit (catalog #163-2105) to familiarize yourself with the
2-D process and operation of the PROTEAN i12 IEF cell. For more details about performing 2-D
electrophoresis, please refer to bulletin 2651, 2-D Electrophoresis for Proteomics: A Methods and Product
Manual.
Fig. 1.5. IEF workflow overview.