Bio-Rad PROTEAN® i12™ IEF System - Manual (Page 28)

Chapter 4 Creating and Editing Protocols
24 | PROTEAN i12 IEF System Instruction Manual 24 |
4.2 Screen Details
Screen Details/Procedure
Main Edit — opens the Select Template screen with options for editing an
existing protocol
Create — opens the New Protocol screen with options for creating
a protocol
Select Template Used to select the protocol that will serve as the template (the
protocol you wish to edit).
Select the memory device, the folder, then the protocol. The Edit
Protocol screen opens, displaying the name and parameters of the
selected protocol.
New Protocol
Edit Protocol
Displays the steps and settings for a new or template protocol: the
New Protocol screen displays a single step, and the Edit Protocol
screen displays steps in a saved protocol. Press Add Step Above or
Add Step Below to add steps as needed (a protocol can contain up
to ten steps).
Touch a cell in the table to edit the settings for the parameters listed
(see Appendix C, IEF Protocols for more details):
Voltage — 0 V or 50–10,000 V
Gradient:
■
Rapid — voltage limited by the set current value
■
Linear — voltage increases in a linear fashion from the starting
voltage to the maximum set voltage. Linearity is not achieved if the
current limit is reached before the required voltage is reached
■
Gradual — bases the voltage change on a delayed voltage
ramping algorithm that gradually increases over the time specified
■
Hold — maintains the voltage, recommended at a field strength
of ~50 V/cm of strip length, until the run is stopped manually. Can
be used as the final step to prevent protein diffusion when IEF is
complete. Steps cannot be added beyond the hold step
µAmps — current (0–100 µA)
Time/VHr — 00:01–99:59 hr or 1–999,999 V-hr
Units — toggle between HH:MM and Volt Hr; previously entered
values reset to 00:01 and 1, respectively
OK — opens the Save Protocol As screen
Bio-Rad protocols cannot be overwritten. Save any changes as
a new protocol.