Bio-Rad PROTEAN® i12™ IEF System - Manual (Page 23)

Chapter 3 Running a Protocol
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Screen Details/Procedure
View Protocol Displays the protocol parameters for the selected protocol.
Back — returns to the Lanes Assigned screen
Run Settings Displays the name of the run data file and offers options for setting
the focusing temperature and conditions for a global rehydration step.
It also offers links to options for entering sample and IPG strip details.
The 12 lane numbers appear, with active lanes listed in boldface and
unassigned lanes grayed out.
To enter or change the name of the run data file, select the text box
next to Start Run to access the keyboard and enter the name.
Focusing Temp. Deg. C — sets the temperature for IEF (10–25ºC,
default 20ºC)
Rehydration — select Yes to program a rehydration step
Rehydration parameters:
■
Hrs — rehydration time (0–99:59 hr, default 12 hr)
■
Volts — rehydration voltage (0 or 50–100 V, default 0 V)
■
Temp. Deg. C — rehydration temperature (10–25ºC, default 20ºC)
■
Pause after rehydration — select Yes if a pause is required for
insertion of electrode wicks
Start — starts the run
Strip Details — opens the Strip Details screen
Sample Details — opens the Sample Details screen
Protocol details, data points, and sample and IPG strip details for
each assigned lane are included in the run data file. Run data files
with the same name are overwritten if saved in the same folder. When
the run is complete, the option to rename the run data file and select
a location will be available. The default storage location for a run data
file is Internal Drive/Bio-Rad Data.
To create and edit protocols, use the Create or Edit option in
the Main screen (see Chapter 4).