Bio-Rad PROTEAN® i12™ IEF System - Manual (Page 7)

Chapter 1 PROTEAN IEF System
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1.1.2 Accessories
The PROTEAN i12 IEF system includes the accessories listed in Table 1.2 and shown in Figure 1.3.
Table 1.2. Accessories for the PROTEAN i12 system. Accessories not included may be purchased separately (see Appendix G,
Ordering Information).
Component Quantity Description
Included with the PROTEAN i12 IEF System
i12 focusing trays
1 each For IEF (and rehydration) of 1–12 IPG strips; each tray includes 2 IPG
with IPG strip retainers (7, 11, and 17 cm) strip retainers to maintain contact between the IPG strip and
electrodes for IEF with the gel-side down configuration
Electrode assemblies 1 set each Positive (+) and negative (-) electrode assemblies; provide contact
between each IPG strip and its power supply
i12 rehydration/equilibration trays 25 each Disposable trays for passive rehydration and equilibration of IPG
(7, 11, and 17 cm) strips
Electrode wicks 100 gel-side up Collect salts and other charged impurities as well as proteins with
500 gel-side down isoelectric points (pI) outside the pH range of the IPG strip (use is
recommended)
ReadyStrip ™ IPG strips 12 each Medium for isoelectric separation of proteins
pH 3–10 (7, 11, and 17 cm)
Mineral oil 500 ml For overlay of IPG strips to prevent dehydration
Forceps 2 pair For manipulation of IPG strips
Cleaning brushes Set of 2 For cleaning the focusing tray and electrode assemblies
Cleaning concentrate 1 L For cleaning the focusing tray and electrode assemblies
USB flash drive 2 Memory data storage device integrated with a USB interface; for
storage and transfer of data from the PROTEAN i12 IEF cell
Stylus 3 Used to manipulate touch screen (user interface)
Leveling bubble 1 Indicates whether the IEF cell is level
Power cord 1 Connects the IEF cell to a power source
ReadyPrep ™ rehydration buffer 10 ml For rehydration and sample loading of IPG strips
Not Included with the PROTEAN i12 IEF System
i12 focusing trays
(13, 18, and 24 cm) For IEF (and rehydration) of 1–12 IPG strips;
each tray includes 2 IPG strip retainers
i12 rehydration/equilibration trays (13, 18, and 24 cm) Disposable trays for passive rehydration and
equilibration of IPG strips
Sample cup holder and sample cups For cup loading of samples
Fig. 1.3. PROTEAN i12 accessories.
i12 rehydration/equlibration trays
Electrode wicks
i12 focusing trays
Electrode assemblies
Cleaning brushes
Styluses
USB flash drive
Leveling bubble
IPG strip retainers
Forceps
Mineral oil
Cleaning concentrate
ReadyStrip IPG strips
ReadyPrep rehydration buffer