Bio-Rad PROTEAN® i12™ IEF System - Manual (Page 40)

Chapter 7 Troubleshooting
36 | PROTEAN i12 IEF System Instruction Manual 36 |
Problem Cause Solution
No current in a lane Poor contact between IPG strip
and electrode Make sure the gel side of the IPG
strip is in direct contact with the
electrode. For the gel-side down
configuration, make sure to
use the IPG strip retainers. See
Chapter 2 for proper placement
of IPG strips
No IPG strip in lane Make sure that the lanes
selected for the run contain IPG
strips before starting a run
Incomplete wetting of electrode
wick Wet the electrode wicks
completely as instructed in
Chapter 2
Incomplete rehydration of IPG
strip Check the rehydration volumes
and times for the IPG strips used
No current in any lane No contact between electrode
assembly and IPG strips Make sure:
■
Electrode assembly is properly
seated in focusing tray
■
IPG strips are positioned
correctly, (for example, that
gel is in direct contact with the
electrode)
No contact between electrode
assembly and instrument Make sure:
■
Gold contact pin of negative (-)
assembly is in direct contact
with cathode bar on instrument
■
Positive (+) assembly is
completely inserted into anode
of instrument
No IPG strips in lanes Make sure IPG strips are
positioned in lanes before
starting a run
No conductivity in IPG strips Make sure IPG strips are
rehydrated with correct reagents
Table 7.1. Troubleshooting guide.