Bio-Rad PROTEAN® i12™ IEF System - Manual (Page 51)

Appendix C IEF Protocols
PROTEAN i12 IEF System Instruction Manual | 47 | 47
Step Voltage (V) Ramp Time Units
1 8,000 Rapid 26,000 Volt Hr
2 750 Hold
Step Voltage (V) Ramp Time Units
1 250 Rapid 0:20 HH:MM
2 8,000 Gradual 1:00 HH:MM
3 8,000 Rapid 26,000 Volt Hr
4 1,500 Hold
Step Voltage (V) Ramp Time Units
1 8,000 Rapid 20,000 Volt Hr
2 750 Hold
Step Voltage (V) Ramp Time Units
1 250 Rapid 0:20 HH:MM
2 8,000 Gradual 1:00 HH:MM
3 8,000 Rapid 32,000 Volt Hr
4 750 Hold
Step Voltage (V) Ramp Time Units
1 250 Rapid 0:20 HH:MM
2 8,000 Gradual 1:00 HH:MM
3 8,000 Rapid 40,000 Volt Hr
4 750 Hold
Step Voltage (V) Ramp Time Units
1 8,000 Rapid 29,000 Volt Hr
2 750 Hold
Step Voltage (V) Ramp Time Units
1 250 Rapid 0:20 HH:MM
2 8,000 Gradual 1:00 HH:MM
3 8,000 Rapid 29,000 Volt Hr
4 750 Hold
11cm pH5.5–6.7
11cm pH6.3–8.3
11cm pH7–10 R
11cm pH7–10 G
11cm pH3–10 R
11cm pH3–10 NL R
11cm pH4–7 R
11cm pH5–8 R
11cm pH3–10 G
11cm pH3–10 NL G
11cm pH4–7 G
11cm pH5–8 G
11cm pH3–6 R
11cm pH3.9–5.1
11cm pH4.7–5.9
Step Voltage (V) Ramp Time Units
1 250 Rapid 0:20 HH:MM
2 8,000 Gradual 1:00 HH:MM
3 8,000 Rapid 20,000 Volt Hr
4 750 Hold
11cm pH3–6 G