Bio-Rad PROTEAN® i12™ IEF System - Manual (Page 3)

PROTEAN i12 IEF System Instruction Manual | iii
Table of Contents
Chapter 1 PROTEAN ® i12 ™ IEF System 1
1.1 System Components 2
1.1.1 PROTEAN i12 IEF Cell 2
1.1.2 Accessories 3
1.2 Unpacking and Setup 5
1.3 Workflow 5
Chapter 2 Basic Operation 7
2.1 Setup 7
2.1.1 i12 ™ Focusing Tray and Electrode Assemblies 7
2.1.2 Connecting the Electrodes 8
2.2 IPG Strip Rehydration and Sample Application 9
2.2.1 IPG Strip Rehydration in the Rehydration/
Equilibration Tray Followed by IEF 9
2.2.2 IPG Strip Rehydration in the Focusing Tray
Followed by IEF 13
2.3 Starting the Run 14
Chapter 3 Running a Protocol 15
3.1 Workflow 16
3.2 Screen Details 18
Chapter 4 Creating and Editing Protocols 23
4.1 Workflow 23
4.2 Screen Details 24
Chapter 5 Setting Defaults and Managing
Folders 27
5.1 Setting Default Parameters (Settings) 27
5.2 Managing Files and Folders (Files) 28
5.2.1 Navigating the Files Structure 28
5.2.2 Copying Files 29
Chapter 6 Data Export and Analysis 31
6.1 File Types 31
6.2 Export to Microsoft Excel Software 31
6.3 Export to PROTEAN i12 Reporter
(www.i12Reporter.com)
32
Chapter 7 Troubleshooting 35
Chapter 8 Cleaning and Maintenance 39
Appendix A
Reagent and Sample Preparation
40
Appendix B
Sample Loading Methods and Running
Configurations 42
Appendix C
IEF Protocols
44
Appendix D
IEF for Peptide Fractionation Prior to LC-MS 50
Appendix E
References 51
Appendix F
Specifications 52
Appendix G
Ordering Information
53