Bio-Rad PROTEAN® i12™ IEF System - Manual (Page 5)

PROTEAN i12 IEF System Instruction Manual | 1
PROTEAN
®
i12
™
IEF
System
1
The PROTEAN i12 IEF system (Figure 1.1) is used for isoelectric focusing (IEF)
on immobilized pH gradient (IPG) strips for the first dimension of two-dimensional
(2-D) electrophoretic protein analysis. The PROTEAN i12 IEF cell can run 1–12
IPG strips in 7, 11, 13, 17, 18, and 24 cm focusing trays. Each channel in the i12
™
focusing tray is powered by its own power supply, enabling precise control over
each IPG strip. This makes it possible to run different sample types, different
gradients, and multiple protocols all at the same time.
The i12 focusing trays and electrode assemblies accommodate all possible
gel configurations (gel-side up or down, with or without electrode wicks). It
also allows sample loading either by inclusion in the rehydration solution (in-gel
loading) or with sample cups (sample cup loading).
The cell is fully programmable from the user interface; connection to an external
computer is not required. Each protocol can contain up to ten steps in which
voltage, manner of voltage ramping, current, and duration (hr or V-hr) are defined.
Fig. 1.1. PROTEAN i12 IEF system. The system includes the PROTEAN i12 IEF cell and numerous accessories.