Bio-Rad PROTEAN® i12™ IEF System - Manual (Page 24)

Chapter 3 Running a Protocol
20 | PROTEAN i12 IEF System Instruction Manual 20 |
Screen Details/Procedure
Strip Details
Sample Details
Used to enter information about the IPG strips (pH range, length, lot,
etc.) and samples in the assigned lanes. Press an assigned lane to
select it and then press Enter Text.
Enter Text — opens the keyboard used to enter the details
Clear All Lanes — removes all entries
OK — accepts the entered details and returns to the Run Settings
screen. The entered strip detail is included with the saved run data file
Save Sample — saves the entered sample details in a specified
location and folder. Sample details are saved to the lane that was
specified
To import an existing sample detail file:
1. Press Import Sample to access previously saved sample detail
files.
2. Select the location and the sample detail file, then press Load
Sample. The sample details populate the assigned lanes.
Run Screens
Rehydration in Progress Displays the rehydration conditions and the time remaining during
rehydration. When the rehydration time has elapsed, the IEF run
starts unless a pause is selected. If a rehydration pause is selected,
the message, Rehydration Completed appears.
Run Protocol — terminates rehydration and starts step 1 of the
protocol(s) in the assigned lane(s)
Back — terminates rehydration and returns to the Run Settings
screen
Running Displays active parameter values for each assigned lane, the time
remaining for the active step, and the total accumulated V-hr for
the run. View Protocol, View Data, and View Graph open the
respective screens.
Pause — pauses the run and opens the System Paused screen,
where the run may be continued or terminated
End Run — terminates the run and opens the End Of Run screen