Bio-Rad PROTEAN® i12™ IEF System - Manual (Page 12)

PROTEAN i12 IEF System Instruction Manual 8 |
Chapter 2 Basic Operation
Fig. 2.1. Placement of an electrode assembly onto
the i12 focusing tray.
2.1.2 Connecting the Electrodes
1. Position the assembly on the Peltier platform. Use the positioning guides and stops on the platform and
focusing tray as guides (Figure 2.2, see inset).
2. Slide the assembly toward the positive (+) end until the positive (+) electrode assembly is completely
inserted. When the the tray positioning stop reaches the positioning guide on the platform, the assembly
is seated correctly (Figure 2.2).
3. Make sure the negative pin on the negative (-) electrode is in direct contact with the metal gounding strip
on the instrument (Figure 2.2).
Fig. 2.2. Connecting the electrodes.
Release clip with green tab
Positive (+) electrode assembly
Release clip with green tab
Orientation
pin
Focusing tray
IPG strip positioning stop
Channel
Tray positioning
stop
Tray positioning
guide
Platform
positioning guide
Platform
positioning guide
Tray positioning
stop
Metal grounding strip
Negative pin
Instrument
platform
Positioning the tray