Bio-Rad PROTEAN® i12™ IEF System - Manual (Page 50)

Appendix C IEF Protocols
46 | PROTEAN i12 IEF System Instruction Manual 46 |
Bio-Rad Protocols
(All protocols have a 50 μA current limit, though 100 μA is possible.)
Step Voltage (V) Ramp Time Units
1 4,000 Rapid 15,000 Volt Hr
2 500 Hold
Step Voltage (V) Ramp Time Units
1 250 Rapid 0:15 HH:MM
2 4,000 Gradual 1:00 HH:MM
3 4,000 Rapid 15,000 Volt Hr
4 500 Hold
Step Voltage (V) Ramp Time Units
1 4,000 Rapid 10,000 Volt Hr
2 500 Hold
Step Voltage (V) Ramp Time Units
1 250 Rapid 0:15 HH:MM
2 4,000 Gradual 1:00 HH:MM
3 4,000 Rapid 20,000 Volt Hr
4 500 Hold
Step Voltage (V) Ramp Time Units
1 250 Rapid 0:15 HH:MM
2 4,000 Gradual 1:00 HH:MM
3 4,000 Rapid 25,000 Volt Hr
4 500 Hold
Step Voltage (V) Ramp Time Units
1 4,000 Rapid 16,000 Volt Hr
2 500 Hold
Step Voltage (V) Ramp Time Units
1 250 Rapid 0:15 HH:MM
2 4,000 Gradual 1:00 HH:MM
3 4,000 Rapid 16,000 Volt Hr
4 500 Hold
7cm pH5.5–6.7
7cm pH6.3–8.3
7cm pH7–10 R
7cm pH7–10 G
7cm pH3–10 R
7cm pH3–10 NL R
7cm pH4–7 R
7cm pH5–8 R
7cm pH3–10 G
7cm pH3–10 NL G
7cm pH4–7 G
7cm pH5–8 G
7cm pH3–6 R
7cm pH3.9–5.1
7cm pH4.7–5.9
Step Voltage (V) Ramp Time Units
1 250 Rapid 0:15 HH:MM
2 4,000 Gradual 1:00 HH:MM
3 4,000 Rapid 20,000 Volt Hr
4 500 Hold
7cm pH3–6 G