Bio-Rad PROTEAN® i12™ IEF System - Manual (Page 8)

Chapter 1 PROTEAN i12 IEF System
4 | PROTEAN i12 IEF System Instruction Manual 4 |
i12 Focusing Tray
Channels in the i12 focusing tray (Figure 1.4) hold IPG strips for IEF. Each focusing tray accommodates up
to 12 IPG strips. Separate trays are available for 7, 11, 13, 17, 18, and 24 cm IPG strips. For flexibility, the
PROTEAN i12 focusing tray and electrode assemblies accommodate all run configurations: gel-side down
or up, with in-gel sample loading or sample cup loading. Each focusing tray holds one negative (-) and one
positive (+) electrode assembly. Focusing trays can also be used for rehydration of IPG strips.
Electrode Assemblies
The electrode assemblies (Figure 1.4) each include 12 individual sets of negative and positive electrodes. The
assemblies attach to the focusing tray to provide contact between each IPG strip and its power supply. They
accommodate all focusing tray sizes.
Fig. 1.4. PROTEAN i12 focusing tray and electrode assemblies.
Negative (-) electrode assembly
Release clip with green tab
Positive (+) electrode assembly
Release clip with green tab
Orientation pin
Release clip
with green tab
Release clip
with green tab
Focusing tray
Tray positioning
stop
IPG strip positioning stop
Channel