Bio-Rad PROTEAN® i12™ IEF System - Manual (Page 53)

Appendix C IEF Protocols
PROTEAN i12 IEF System Instruction Manual | 49
Step Voltage (V) Ramp Time Units
1 10,000 Rapid 60,000 Volt Hr
2 1,500 Hold
Step Voltage (V) Ramp Time Units
1 250 Rapid 0:30 HH:MM
2 10,000 Gradual 2:00 HH:MM
3 10,000 Rapid 60,000 Volt Hr
4 1,500 Hold
Step Voltage (V) Ramp Time Units
1 10,000 Rapid 44,000 Volt Hr
2 1,500 Hold
Step Voltage (V) Ramp Time Units
1 250 Rapid 0:30 HH:MM
2 10,000 Gradual 2:00 HH:MM
3 10,000 Rapid 70,000 Volt Hr
4 1,500 Hold
Step Voltage (V) Ramp Time Units
1 250 Rapid 0:30 HH:MM
2 10,000 Gradual 2:00 HH:MM
3 10,000 Rapid 88,000 Volt Hr
4 1,500 Hold
Step Voltage (V) Ramp Time Units
1 10,000 Rapid 63,000 Volt Hr
2 1,500 Hold
Step Voltage (V) Ramp Time Units
1 250 Rapid 0:30 HH:MM
2 10,000 Gradual 2:00 HH:MM
3 10,000 Rapid 63,000 Volt Hr
4 1,500 Hold
24cm pH5.5–6.7
24cm pH6.3–8.3
24cm pH7–10 R
24cm pH7–10 G
24cm pH3–10 R
24cm pH3–10 NL R
24cm pH4–7 R
24cm pH5–8 R
24cm pH3–10 G
24cm pH3–10 NL G
24cm pH4–7 G
24cm pH5–8 G
24cm pH3–6 R
24cm pH3.9–5.1
24cm pH4.7–5.9
Step Voltage (V) Ramp Time Units
1 250 Rapid 0:30 HH:MM
2 10,000 Gradual 2:00 HH:MM
3 10,000 Rapid 44,000 Volt Hr
4 1,500 Hold
24cm pH3–6 G