Luminex xPONENT 3.1 Rev 2 (IVD) - Manual (Page 30)

entire column or row, or simply click and highlight different wells and then click a command
below to assign that command to all the highlighted wells.
• Unknown (U): Yellow
• Background (B): Purple
• Control (C): Red
• Standard (S): Green
The Delete and Start at Well commands are also available to assign as well commands.
Delete removes the well command for the selected well. The Start at Well command enables
you to begin acquisition at a well other than A1.
NOTE: You should first delete all standards from the plate layout if any of
the standards need to be rearranged, and all controls from the plate
layout if any of the controls need to be rearranged.
NOTE: Wells and commands you assign to the protocol plate layout are
saved into the protocol settings and execute each time you use the
protocol to run a batch. Standards and controls associated with a
given protocol typically remain constant, while the number of
unknown wells often vary. You can assign a specific number of
unknown wells to the plate when setting up a batch.
Commands - Assigns maintenance commands that run before or after single wells, ranges of
wells, and rows or columns of wells. Select a well, and then select one of the following
commands:
• Alcohol Flush
• Prime
• Sanitize
• Soak
• Wash
NOTE: These commands are run from a reservoir in the Off-Plate Area.
After selecting a maintenance command, click one of the following buttons:
• Before Well - The system performs the command before acquiring the well. An open dot
appears in the upper left corner of the selected well on the plate image. The command
appears in the command sequence list before the selected well.
• After Well - The system performs the command after acquiring the well. A closed dot
appears in the lower right corner of the selected well on the plate image. The command
appears in the command sequence list after the selected well.
• Clear Command - Select a well and click Clear Commands to remove all maintenance
commands from the well.
Plate - Specifies the plate to display in the plate image in the list. Add Plate adds a new plate
to the batch, and Delete Plate deletes the plate highlighted in the list.
Direction - Specifies the direction to run the plate commands. Select either horizontally or
vertically. The selected direction also dictates how wells are added to the plate when
assigning multiple unknowns, standards, and controls at one time.
Plate Navigation - Displays a smaller plate image for the current batch.
xPONENT ® 3.1 Rev. 2 Software User Manual
20
For In Vitro Diagnostic Use