Luminex xPONENT for MAGPIX 4.2 - Manual (Page 73)

• Control Range - High - The highest value for an assay control used to determine pass/
fail criteria for an assay.
• Normalized Net Median - For each analyte in a well the Normalized Net Median (NNM)
= (net median of analyte) / (net median of normalization bead)
• Units - The unit of measure for an analyte, for example, pg/mL.
• Analyte - Contains a list of analytes run in the batch. Select an analyte to view all statistics
for that analyte.
• Well(s) to View
• Current Well - Displays the statistics of the well currently being displayed (This changes
to Displayed Well if viewing a batch using the Open button of the Saved Batches tab).
• Single Step - Instrument analyzes one well at a time. Click to turn function on or off.
This is useful to run prior to running an entire batch to confirm that the system is set up
correctly.
• Results pane - Displays statistics related to the batch.
• Use the up, down, left, and right arrow buttons to move through the table, or use the
scroll bars.
• Plate - Select the plate you want to view, if there is more than one plate.
CAUTION: If using multiple plates, ensure that plates are used in the proper
order. Failure to do so can result in inaccurate data and test
results.
• Well Report pane - This pane displays a representation of the plate and the status of
acquired wells. Each well displays one of three possible states:
• Yellow - Well acquired, but the system detects a possible problem (select the Log tab
for more information).
• Green - Well acquired successfully.
• Red - Well acquisition unsuccessful, the system might have stopped, depending on the
circumstances (select Log tab for more information).
• Dot Plot pane - The default location of the dot plot is the lower-right section of the Current
Batch tab. The dot plot is a graphical display of real-time data collection. The dot plot
default display when using 1 to 50 beads shows Classification 1 (CL1) and Classification
2 (CL2). Click within the dot plot to open Display Mode, with its two options:
• Logarithmic. This is the default option.
• Linear
• Log pane - This displays a log of system processes. Log entries indicating warnings are
highlighted in yellow; errors are highlighted in red. Other log entries are not highlighted.
The log includes the following information:
• Date
• Message
• Code
• Save Image - Opens a Save As dialog box to save a screen capture.
• Details - Opens the Results tab, enabling more analysis and results.
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