Luminex xPONENT for MAGPIX 4.2 User Manual

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• Import List - Opens the Open dialog box to import an existing command sequence list.

• Replicate Count - Defines a quantity of replicate sets from one to nine.

NOTE: Replicate count selection must be made before adding a well

command.

• Grouping - Selects the sequence in which the replicates are laid out in plate wells.

NOTE: Grouping selection must be made before adding a well command.

The options are:

• 123123123. . . Lays out one of each replicate set at a time in numerical order.

• 111222333. . . Lays out all the replicates in a set before moving on to the next set in

numerical order.

You can assign the following well commands. Each command is associated with a color.
You can click and drag to highlight a series of wells, click a column or row header to
highlight the entire column or row, or click and highlight different wells and then click a
command below to assign that command to all the highlighted wells.

• Unknown (U): Yellow

• Background (B): Purple

• Control (C): Red

• Standard (S): Green

The Delete and Start at Well commands are also available to assign as well commands.
Delete removes the well command for the selected well. The Start at Well command enables
you to begin acquisition at a well other than A1.

NOTE: Before adding well commands, delete all standards from the plate

layout if any of the standards need to be rearranged. Delete all
controls from the plate layout if any of the controls need to be
rearranged.

NOTE: Wells and commands you assign to the protocol plate layout are

saved into the protocol settings and execute each time you use the
protocol to run a batch. Standards and controls associated with a
given protocol typically remain constant, while the number of
unknown wells often varies. You can assign a specific number of
unknown wells to the plate when setting up a batch.

Commands and Routines - Allows you to add and delete commands and routines, and to
create pre and post batch routines. Select a well and then select the appropriate command:

• Add

• Delete

• Pre Batch Routine

• Post Batch Routine

NOTE: If you select a routine you created, that routine must also exist on

any system to which you import this protocol. The system displays
an error when attempting to run a batch on a system where the
routine does not exist.

Batches Page