Luminex 100 User Manual Version 1.7 - Manual (Page 82)

Luminex 100 User Manual Version 1.7 x MAP Technology
7 - 2 PN 89-00002-00-063 Rev. A
3. While you are waiting for the analyzer and optics system to
warm up - about 30 minutes - check the levels of sheath fluid and
waste fluid. Check that the sheath container cap is tight.
4. Prime the system.
5. Perform an Alcohol Flush with 70% isopropanol or 70%
ethanol.
6. Wash twice with distilled water or sheath fluid. For optimal
performance, Calibrate the system and then Wash four times
with distilled water. See page 5-2 for more information about
calibration.
Shutdown
‹ Perform the shutdown procedure when you finish running
samples.
1. Dispense 1.2 mL of a fresh 10%-20% bleach solution into the
Luminex XYP reservoir. Run a Sanitize command.
2. Run a Soak command with distilled water.
3. Raise the sample probe.
4. Click Eject on the toolbar to eject the plate holder.
5. Remove the microtiter plate and the reservoir.
6. Clean the plate holder and reservoir with a 10%-20% bleach
solution.
7. Replace the reservoir, then click Retract on the toolbar to retract
the plate holder.
8. Loosen the sheath fluid container cap to release pressure in the
system.
9. Turn the analyzer and PC off.
Check Fluid Levels
Check these sheath and waste fluids daily, more often if you run a lot
of samples. Replace the sheath fluid and empty the waste container
as needed. You must manually monitor the sheath fluid and waste
container levels. Release the Luminex 100 analyzer’s pressure by
removing the lid from the sheath fluid container. Add sheath fluid,
filling the sheath fluid container to just below the air intake. Discard
waste by appropriate means. After you add sheath fluid and discard
Note: There is NO sensor to warn
you of low sheath fluid levels. Fill
the sheath fluid container before
the level reaches the sheath outlet
line. There is NO indicator light to
warn you of high waste volume.
Empty the waste container each
time you fill the sheath container.