Luminex 100 User Manual Version 1.7 - Manual (Page 125)

PN 89-00002-00-063 Rev. A A - 3
x MAP Technology emission spectrum
emission spectrum Wavelength range that an excited fluorochrome emits when its
electrons fall from a higher to a lower energy state. Expressed in
nanometers (nm).
event Occurs when the signal processor determines that a particle is being
observed.
Ex/Em A common way of reporting a molecule’s excitation and emission
wavelengths (for example, Ex/Em = 488/520).
excitation spectrum Wavelength range that excites a molecule’s electrons to a higher
energy state. Expressed in nanometers (nm).
flow rate Velocity of a fluid stream. Reported as µL/second or µL/minute in
microparticle analyzers.
fluorescence Light emission that occurs when the electrons of a fluorochrome
drop to a lower energy state.
fluorochrome A fluorescent molecule.
fluorophore See fluorochrome.
folder A directory or subdirectory containing details of an experiment such
as PMT settings, gates, data, etc. The user selects the folder that
contains data for an experiment. The folder name is also the name for
that experiment and contains the .CSV and run files for that
experiment.
gate A user-defined parameter in the histogram represented by red dotted
lines on the graph and numerically indicated in parentheses in the
lower left corner of the graph. The gate is used to discriminate single
beads from doublets. Draw a gate on the histogram only when the x
axis is set to Doublet Discriminator.
histogram Provides a graphical display of single parameter data.
immunofluorescence A technique which uses a covalently linked fluorochrome-antibody
complex to detect or quantify a particular antigen.
inject rate The rate at which a sample is injected into the sheath fluid stream.
labeled antigen An antigen that has been conjugated to another molecule (typically
avidin, biotin, or fluorochrome), for use in a reporter system.