Luminex 100 User Manual Version 1.7 - Manual (Page 46)

Luminex 100 User Manual Version 1.7 x MAP Technology
5 - 6 PN 89-00002-00-063 Rev. A
7. Fill the reservoir with a solution of 70% isopropanol or 70%
ethanol. Click Retract on the toolbar to retract the plate holder.
8. Click Alcohol Flush, then click OK. The alcohol flush removes
air from the fluidics. This takes about 5 minutes.
9. Click Calibrate on the main screen. The Start Calibration dialog
box opens.
Figure 5-7. Start Calibration Dialog Box
10. Enter your name in the Operator text box.
11. Select the Product / Lot Number for the xMAP classification
calibration and reporter microspheres.
12. Click OK. The Calibration dialog box opens.
Figure 5-8. Calibration Dialog Box
13. Make sure Classification is selected, and click Start.
The progress bar shows the calibration progress, which should
take less than two minutes. If the status changes to “Sample
Empty” before calibration completes, a “Calibration Failed”
message appears. Click OK and restart the process. When
calibration finishes for the classification microspheres, a