Luminex 100 User Manual Version 1.7 - Manual (Page 123)

PN 89-00002-00-063 Rev. A A - 1
Glossary
aggregated
microspheres Two or more xMAP microspheres that have adhered together.
Alexa 532 Fluorochrome used for reporter channel with Ex/Em=531/554.
(product of Molecular Probes, Inc.)
ambient temperature The temperature of the surrounding environment.
analyte A substance that is detected through assay analysis. Each test or
unique xMAP microsphere type will test for a specific analyte.
assay A quantitative measurement of a particular compound.
background (noise) That portion of a bead set result that can be attributed to excess
reporter molecules in the solution, nonspecific binding, or
fluorescent spillover from another fluorochrome.
bead Shorthand terminology for a xMAP microsphere.
bead control A sample in an assay or test that contains only conjugated xMAP
microspheres; no reporter or specimen.
bead ID Identification number for an xMAP microsphere set.
bead set See Region.
calibration A process used to normalize the settings for the reporter channel,
both classification channels, and the doublet discriminator channel.
Calibration ensures optimal and consistent xMAP microsphere
classifications and reporter readings.
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