Luminex 100 User Manual Version 1.7 - Manual (Page 111)

PN 89-00002-00-063 Rev. A 9 - 1
Protocols
Updates or additions to these protocols are posted on the Luminex
website at
http://luminexcorp.custhelp.com .
The protocols in this section are presented in the following order:
‰ Two-Step Carbodiimide Coupling of Protein to xMAP
Carboxylated Microspheres
‰ One-Step Carbodiimide Coupling of Oligonucleotides to
Luminex Carboxylated Microspheres
‰ Binding Biotin-conjugated Molecules to LumAvidin
®
-Modified
Microspheres
xMAP Microsphere
Handling
Microsphere Dispersion
Luminex xMAP microspheres settle and aggregate if left
undisturbed. You should always ensure that they are evenly
distributed before dispensing. Gentle vortexing and sonication are
effective methods of mixing for most coated xMAP microsphere
preparations. After dispersion, xMAP microspheres generally remain
in solution for about one hour. To maintain bead concentration within
the stock xMAP microsphere volumes (1 ml, 4 ml, or 16 ml), we
suggest that you follow these guidelines.
For complete resuspension of the 1 ml stock vials, Luminex
recommends that you:
1. Allow the contents of the vial to equilibrate to room temperature.
2. Centrifuge for 1-2 minutes at 10,000 x g.
9