ChemoMetec NC-100 - Manual (Page 59)

7 Troubleshooting
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7 Troubleshooting
Occasionally difficulties with the NucleoView software may occur. In the following a short
description and suggested action is given.
7.1 Quality of the sample
Through the CM file image it is possible visually to assess the quality of the sample
preparation and thereby the reliability of the measurement by looking at the “star ry sky”.
The measurement is reliable if the stars or white dots are distributed separately and
evenly over the image. Naturally, this rule only applies when a representative sample has
been drawn from the original cell suspension.
Be aware of large objects in the image. These objects can be external material, such as
dust or a piece of hair. These objects are ignored in the image analysis, so the obtained
result correctly reflects the number of individual c ells in the sample. On the other hand, it
is important to be aware of these objects, since if it is a cell aggregate or a clump this
might indicate that the sample preparation has been sub optimal and the quality of the
estimate of cell concentration may b e compromised.
7.2 Data not received
If NucleoView does not receive data from the NucleoCounter, please verify that the USB
cable is connected correctly to both the NucleoCounter and the comp uter. Then look at
the task bar on the computer to see if the NC Receiver is started.
Figure 45 NC Receiver icon is Green on the Windows task bar when connected and Grey
when disconnected
The NC Receiver must be started to receive data from the NucleoCounter. If the NC
Receiver is not connected, quit and restart NucleoView. NC Receiver should then appear
on the task bar.
Generally the NC Receiver has two states in the Instrument Status field when it is started:
Not connected
Connected