ChemoMetec NC-100 - Manual (Page 26)

4 Measurement tab
16
4.1 Concentration of cells in the NucleoCassette
TM
The estimated cell concentration of the cell lyzate in the NucleoCassette is displayed at the
top of the window in a field labeled “Concentration of cells in NucleoCassette”. The cell
concentration is given as cells per mL.
Most often the cell suspension has been diluted with the Lysis and Stabilizing buffers
before the analysis. To obtain the concentration of cells in the original cell suspension the
result has to be corrected using the multiplication factor (see chapter 4.2 Concentration of
cells in suspension and Multiplication factor below).
4.2 Concentration of cells in suspension and Multiplication factor
The result of the cell counts adjusted for the appropriate dilutions (using the
multiplication factor) is displayed near the bottom of the window using large types. The
multiplication factor is used to correlate the observed cell concentration in the
NucleoCassette to the concentration of cells in the original cell suspension. Thus, the
concentration of cells in the NucleoCassette times the multiplication factor equals the
concentration of cells in the original cell suspension.
When there is a checkmark in the field next to “Use multiplication factor” (as shown in
figure 13) the multiplication factor can be changed manually. The multi plication factor can
be changed after the CM file is received, either by entering a new value in the field below
“Multiplication factor”. The factory set default value of the multiplication factor is 3 and
changes to this is described in section 6.6 Default settings for NucleoViewTM.
When the checkmark next to “Use multiplication factor” is removed (see figure 14) it
becomes possible to enter information about the various volumes used for the analyses.
NucleoView then calculates the multiplication factor according to the formula shown
below.
MF The multiplication factor used for correction of the concentration of cells in the
NucleoCassette.
MF
EX
The external multiplication factor e.g. from a pre -dilution.
V
LB
The volume of the Lysis buffer.
V
SB
The volume of the Stabilizing buffer.
V
S
The volume of the sample.