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Iability, Oncentration of viable cells, Cells – ChemoMetec NC-100 User Manual

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5 Viability tab

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shown immediately. If comments have been added to a file this will be shown next to the

name of the CM file.

If the first file is selected as the total cell count, no file is automatically selected as the

non-viable cell count and only the file list is shown (the information about the file and the

results disappear). Select the file that is the non -viable cell count manually and the

information about the file and results will be shown.

If the same file is chosen for the total cell count and the non -viable cell count an error

message will appear, see figure 24. Click OK and NucleoView changes the non-viable file to

be the file prior to the file chosen for the total cell count.

Figure 24 Error message when the same files are chosen for the total and non -viable cell

count.

To choose results from another session click the “Browse”-button. Follow the directions

described in chapter 4.5 Browse and Today.

To save the result of a viability calculation click the “Save result” -button. The result will be

saved instantly and no dialogue will pop up. After a result has been saved it is not possible

to delete it again.

The saved results can be printed or exported to a tabulator-separated file, which can be

imported into several applications such as word processors or spreadsheets. See chapter

6.3 Viability report and chapter 6.4 Import data into Excel.

5.2

Viability (%) and Concentration of viable cells (cells/mL)

Once the two files have been selected for the viability analysis the resul ts are present at

the bottom of the Viability tab. The results are the Viability in % and the Concentration of

viable cells in cells/mL. The representation of the results of the calculations follows the

result mode for the total cell count. The result mode is selected on the NucleoCounter.

Please refer to the NucleoCounter User's Guide for further information on result mode and

calculation of viability.

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