ChemoMetec NC-100 - Manual (Page 32)

4 Measurement tab
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When CM-files are not from today’s session the “Edit ” button is shown. This button must
be activated before changes of the settings can be made, however these changes will only
be saved to the CM-file if accessed using the PC that collected the files, and if the files still
remain in their original folder. The files cannot be changed from other PCs or if they have
been moved from the original folder.
It is not possible to save changes to CM-files generated with versions of
NucleoView prior to version 2.0.
4.8 Collected image and Object intensity histogram
When estimating cell concentration with the NucleoC ounter, an image of the stained
nuclei in the NucleoCassette chamber is collected. This image is transferred to the
computer and NucleoView presents it. The image bears resemblance to a starry sky, where
each of the stars is a stained nucleus. Figure 18 shows an example of an image displayed
by NucleoView.
Figure 18 NucleoView presents the images from the NucleoCounter like a starry sky. Each
“star” represents a stained nucleus.
The NucleoCounter records the image of the NucleoCassette chamber when counting cells.
The image is then processed in the build -in computer of the NucleoCounter to give an
estimate of the cell concentration, counting every star or white dot as a nucleus.
The object intensity histogram is shown below the image of the nuclei, see figure 19 The
object intensity histogram. It shows the distribution of the number of fluorescence signals
(particles) as a function of the intensity of the objects. In other words, the unit of the x -