beautypg.com

Plate map setting procedure, Select mapping mode menu, A. manual mapping manual mapping – Bio-Rad iMark Microplate Absorbance Reader User Manual

Page 39

background image

iMark Microplate Absorbance Reader Instruction Manual

33

Plate Map Setting Procedure

Select Mapping Mode Menu

This menu screen is entered by choosing "Mapping" in the End Point Protocol Menu screen or
the Kinetic Protocol Menu screen. It is used to map the plate format. The Up and Down arrow
keys move the cursor next to the desired item of menu list. The Enter key seals the selection
and changes this screen to the next setting screen.

a. Manual Mapping

Manual Mapping

In this screen, the user can set the plate format manually. Ten well types can be set.

Well types:

The supported well types are:

Well type

Indication on LCD

1. Blank:

B

2. Sample:

X00…X99

3. Standard:

S01…S99

4. In-kit Control: Positive Control

CP0…CP9

5.

Weak Positive Control

CW0…CW9

6.

Negative Control

CN0…CN9

7.

Cutoff Control

CO0…CO9

8. Calibrator

CL0…CL9

9. QC Control:

QC0…QC9

10. Empty well:

-


The key assignments of well type are:

“0/EMP” Empty

well

“5/QC”

Quality

Control

well

“1/SMP” Sample

well

“6/CAL” Calibrator

well

“2/BLK” Blank

well

“7/CP”

Positive Control well

“3/STD”

Standard

well

“8/CN”

Negative Control well

“4/CO”

Cutoff

Control

well

“9/CW”

Weak Positive Control well

Two Edit Modes in Plate Mapping:

There are two editing modes; for well type input mode and for well number input. The
current input mode is displayed at the upper left corner of the screen. When using the
well type input mode, the mode sign is “[F]”. When using the well number input mode,
the mode sign is “■N■”.
The cursor that encloses the editing position of well has two types of enclosures
depending on the mode. For example, “[S01]” accepts the well type keys that be assigned
to the numerical keys and “■S01■” accepts the numerical input.
The mode is easily changed with the Decimal point key.

Key Functions:

The Up, Down, Right and Left arrow keys move the cursor to the desired well. The
movement of the cursor follows the scroll of this screen. The Left arrow key will return
the screen to the preceding screen, or the "select Mapping mode Menu", only if the
cursor is on column #1. At this time, the selection screen appears (see the left figure).
The Decimal point key changes the input mode.
The Numerical keys have two functions; one to input the number and another to select a
well type.
The Enter key checks the consistency between the edited plate map and other parameters,
memorizes the plate map into the protocol and changes this screen back to the preceding
screen. If it finds any inconsistency, a warning message will appear on the screen.

Select Mapping mode
»

■Manual mapping

•Automatic mapping

■N■ 1 2 3 4
A

│■ B■ CN1 S01 S01

B

│ B CN1 S02 S02

C

│ B CN2 S03 S03

[

F] 5 6 7 8

A

│ X11 X12 X13 X14

B

│ X21 X22 X23 X24

C

│ X31 X32 X33[X34]

Discard change?
1:Yes 2:No
Press 1 or 2 key